Journal: International Journal of Molecular Sciences
Article Title: β3-Adrenoreceptor Activity Limits Apigenin Efficacy in Ewing Sarcoma Cells: A Dual Approach to Prevent Cell Survival
doi: 10.3390/ijms20092149
Figure Lengend Snippet: ( A ) Analysis of cellular viability with MTT survival experiment in human A673 ES cells after 24 h of treatment with apigenin (10-20-50 μM); ( B ) Percentage of live cells after Viobility TM Fixable Dyes assay after 24 h of treatment with apigenin (10-20-50 μM); ( C ) The apoptotic effect of apigenin analysed after 24 h of treatment (10-20-50 μM); ( D ) Western Blot analysis of PARP1 enzyme after treatment with apigenin (10-20-50 μM) with β-actin as loading control; ( E ) Percentage of live cells after Viobility TM Fixable Dyes assay and apoptotic effect on healthy lymphocytes after 24 h of treatment with apigenin (10-20-50 μM). Apig: apigenin, ns: not significant. p values for treatments: * p < 0.05, ** p < 0.01 and *** p < 0.001.
Article Snippet: Human Ewing Sarcoma (ES) cells A673 were purchased from the American Type Culture Collection (ATCC ® CRL-1598 TM , Manassas, VA, USA).
Techniques: Western Blot, Control